TY - JOUR T1 - Chronic but not intermittent infection with <em>Pseudomonas aeruginosa</em> is associated with global changes of the lung microbiome in cystic fibrosis JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.01086-2017 VL - 50 IS - 4 SP - 1701086 AU - Sébastien Boutin AU - Simon Y. Graeber AU - Mirjam Stahl AU - A. Susanne Dittrich AU - Marcus A. Mall AU - Alexander H. Dalpke Y1 - 2017/10/01 UR - http://erj.ersjournals.com/content/50/4/1701086.abstract N2 - Chronic pulmonary infection with Pseudomonas aeruginosa that triggers chronic airway inflammation and structural lung damage remains a key risk factor for morbidity and mortality in patients with cystic fibrosis (CF) [1]. Previously, culture-based diagnostics considered airway infections in CF patients as mono-pathogenic, but recent approaches using culture-independent methods showed that bacterial infection of the CF lung involves a polymicrobial community [2–4]. Chronic infection with typical CF pathogens like P. aeruginosa is associated with a decline in lung function and correlates with a decrease of diversity in the lung microbiota. However, the interactions between pathogens and the microbiota remains poorly understood, and it has been hypothesised that dysbiosis of the microbiota may pre-exist and facilitate growth of pathogens such as P. aeruginosa in the CF lung. The aim of the current study was to evaluate the influence of P. aeruginosa infection on the dysbiosis of the microbiota in the CF lung.Chronic but not intermittent P. aeruginosa infection results in dysbiosis of lung microbiota in cystic fibrosis http://ow.ly/cDKO30eOnx5We acknowledge the excellent technical support by Jessica Panitz, Selina Hassel, David Ibbersson and the technical team from Bioquant. We are also grateful to Iris Kühbandner for patient recruitment and sample collection. Author contributions: Conception and design of the study: S. Boutin, S.Y. Graeber, M. Stahl, A.S. Dittrich, M.A. Mall, A.H. Dalpke; acquisition, analysis and interpretation of the data: S. Boutin, S.Y. Graeber, M. Stahl, A.S. Dittrich, M.A. Mall, A.H. Dalpke; drafting the article or revising it critically for important intellectual content: S. Boutin, S.Y. Graeber, M. Stahl, A.S. Dittrich, M.A. Mall, A.H. Dalpke. ER -